Protein synthesis assisted by native chemical ligation at leucine

Ziv Harpaz, Peter Siman, K. S. Ajish Kumar, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

119 Scopus citations

Abstract

(Chemical Equation Presented) Triggering leucine: A new ligation strategy of using β-mercaptoleucine coupled with desulfurization at leucine sites was developed, and its applicability in protein synthesis is presented. The efficiency of our Leu-NCL was examined in several model peptides and utilized for the first total synthesis of HIV-1 Tat protein.

Original languageEnglish
Pages (from-to)1232-1235
Number of pages4
JournalChemBioChem
Volume11
Issue number9
DOIs
StatePublished - 14 Jun 2010

Keywords

  • Desulfurization
  • Leucine
  • Native chemical ligation
  • Peptides
  • Protein synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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