@inbook{59e0fe82d136473fa588244dc816d4ce,
title = "Proteomic Analysis of Non-model Plant Tissues Using Phenol Extraction, Two-Dimensional Electrophoresis, and MALDI Mass Spectrometry",
abstract = "Separation of plant proteins by means of electrophoretic techniques is quite challenging since different compounds typical for plant cells can interfere and/or reduce the effectiveness of the protein isolation. This is particularly problematic for two-dimensional electrophoresis (2-DE). Therefore, it is important to optimize protein extraction and to establish a robust protocol for 2-DE and downstream processing, primarily mass spectrometry (MS) analysis. Here we give a detailed protocol for protein extraction using phenol method, 2-DE, and MALDI-MS analysis.",
keywords = "De novo sequencing, Isoelectric focusing, MALDI-MS/MS, Phenol extraction, SDS-PAGE, Two-dimensional electrophoresis",
author = "\{Peharec {\v S}tefani{\'c}\}, Petra and Mario Cindri{\'c} and Biljana Balen",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-8594-4\_25",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "351--370",
booktitle = "Methods in Molecular Biology",
}