Proteomic analysis of SETD6 interacting proteins

Ofir Cohn; Ayelet Chen; Michal Feldman; Dan Levy

doi:10.1016/j.dib.2016.01.042

Proteomic analysis of SETD6 interacting proteins

Ofir Cohn, Ayelet Chen, Michal Feldman, Dan Levy

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.

Original language	English
Pages (from-to)	799-802
Number of pages	4
Journal	Data in Brief
Volume	6
DOIs	https://doi.org/10.1016/j.dib.2016.01.042
State	Published - 1 Mar 2016

Keywords

Interactome
Mass spectrometry
SETD6

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.dib.2016.01.042

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title = "Proteomic analysis of SETD6 interacting proteins",

abstract = "SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.",

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author = "Ofir Cohn and Ayelet Chen and Michal Feldman and Dan Levy",

note = "Publisher Copyright: {\textcopyright} 2016.",

year = "2016",

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doi = "10.1016/j.dib.2016.01.042",

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T1 - Proteomic analysis of SETD6 interacting proteins

AU - Cohn, Ofir

AU - Chen, Ayelet

AU - Feldman, Michal

AU - Levy, Dan

PY - 2016/3/1

Y1 - 2016/3/1

N2 - SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.

AB - SETD6 (SET-domain-containing protein 6) is a mono-methyltransferase that has been shown to methylate RelA and H2AZ. Using a proteomic approach we recently identified several new SETD6 substrates. To identify novel SETD6 interacting proteins, SETD6 was immunoprecipitated (IP) from Human erythromyeloblastoid leukemia K562 cells. SETD6 binding proteins were subjected to mass-spectrometry analysis resulting in 115 new SETD6 binding candidates. STRING database was used to map the SETD6 interactome network. Network enrichment analysis of biological processes with Gene Ontology (GO) database, identified three major groups; metabolic processes, muscle contraction and protein folding.

KW - Interactome

KW - Mass spectrometry

KW - SETD6

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U2 - 10.1016/j.dib.2016.01.042

DO - 10.1016/j.dib.2016.01.042

M3 - Article

AN - SCOPUS:84956685159

SN - 2352-3409

VL - 6

SP - 799

EP - 802

JO - Data in Brief

JF - Data in Brief

ER -

Proteomic analysis of SETD6 interacting proteins

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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