Protocol for miRNA in situ hybridization on mouse spinal cord

Irit Reichenstein, Eran Hornstein

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

microRNAs (miRNAs) are key posttranscriptional regulators of gene expression and are involved in a variety of processes in the central nervous system. miRNAs play numerous roles in neuro-development and are crucial for the proper homeostatic function of neurons in adults. The detection of miRNAs in specific cell types sheds light on differential regulation of their expression and on the potential functional roles of miRNAs in the brain. In this chapter, we present a simple and robust protocol for the detection of miRNAs by in situ hybridization in formalin-fixed paraffin-embedded spinal cords of mature mice and developing embryos.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages1-10
Number of pages10
DOIs
StatePublished - 1 Jan 2017
Externally publishedYes

Publication series

NameNeuromethods
Volume128
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • In situ hybridization
  • Spinal cord
  • miRNAs
  • microRNA

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry, Genetics and Molecular Biology
  • General Pharmacology, Toxicology and Pharmaceutics
  • Psychiatry and Mental health

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