Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease

Jack S. Cohen; Richard I. Shrager; Mildred Mcneel; Alan N. Schechter

doi:10.1038/228642a0

Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease

Jack S. Cohen, Richard I. Shrager, Mildred Mcneel, Alan N. Schechter

Research output: Contribution to journal › Short survey › peer-review

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Abstract

The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.

Original language	English
Pages (from-to)	642-644
Number of pages	3
Journal	Nature
Volume	228
Issue number	5272
DOIs	https://doi.org/10.1038/228642a0
State	Published - 1 Dec 1970
Externally published	Yes

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title = "Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease",

abstract = "The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.",

author = "Cohen, {Jack S.} and Shrager, {Richard I.} and Mildred Mcneel and Schechter, {Alan N.}",

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T1 - Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease

AU - Cohen, Jack S.

AU - Shrager, Richard I.

AU - Mcneel, Mildred

AU - Schechter, Alan N.

PY - 1970/12/1

Y1 - 1970/12/1

N2 - The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.

AB - The imidazole C2-H resonances in the 220 MHz proton magnetic resonance spectra of staphylococcal nuclease have been identified with the aid of computer curve-fitting. Spectra were fitted to Lorentzian curves for the measurement of peak area, and chemical shifts were fitted to theoretical pH titration curves for the precise determination of pK values. Such a high resolution analysis makes a conformational equilibrium hypothesis involving histidine unlikely in the case of nuclease.

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DO - 10.1038/228642a0

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JO - Nature

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ER -

Proton magnetic resonance studies at 220 MHz of the histidine residues of staphylococcal nuclease

Abstract

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