Proximal colon-derived O-glycosylated mucus encapsulates and modulates the microbiota

Kirk Bergstrom, Xindi Shan, David Casero, Albert Batushansky, Venu Lagishetty, Jonathan P. Jacobs, Christopher Hoover, Yuji Kondo, Bojing Shao, Liang Gao, Wesley Zandberg, Benjamin Noyovitz, J. Michael McDaniel, Deanna L. Gibson, Sepideh Pakpour, Negin Kazemian, Samuel McGee, Courtney W. Houchen, Chinthalapally V. Rao, Timothy M. GriffinJustin L. Sonnenburg, Rodger P. McEver, Jonathan Braun, Lijun Xia

Research output: Contribution to journalArticlepeer-review

108 Scopus citations

Abstract

Colon mucus segregates the intestinal microbiota from host tissues, but how it organizes to function throughout the colon is unclear. In mice, we found that colon mucus consists of two distinct O-glycosylated entities of Muc2: A major form produced by the proximal colon, which encapsulates the fecal material including the microbiota, and a minor form derived from the distal colon, which adheres to the major form. The microbiota directs its own encapsulation by inducing Muc2 production from proximal colon goblet cells. In turn, O-glycans on proximal colon-derived Muc2 modulate the structure and function of the microbiota as well as transcription in the colon mucosa. Our work shows how proximal colon control of mucin production is an important element in the regulation of host-microbiota symbiosis.

Original languageEnglish
Article numberaay7367
JournalScience
Volume370
Issue number6515
DOIs
StatePublished - 23 Oct 2020
Externally publishedYes

ASJC Scopus subject areas

  • General

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