Members of the HSP70 family were purified from electric organ tissue of Torpedo californica by chromatography on an ATP-agarose column. A 70 kDa protein was the major component purified. The identity of this protein as HSP70 was established by Western blotting with monoclonal antibodies raised against purified and recombinant human HSP70. Sequencing of proteolytic fragments revealed that they possessed high sequence homology with a wide spectrum of members of the HSP70 family from bacteria through to humans. Two minor components, which co-purified with the principal 70 kDa band on the ATP-agarose column, may correspond to Torpedo BiP and to the stress-induced version of HSP70.
|Number of pages||7|
|Journal||Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology|
|State||Published - 1 Jan 1995|
- Electric organ
- Heat shock proteins
ASJC Scopus subject areas
- Molecular Biology