Purification of the M. magneticum strain AMB-1 magnetosome associated protein MamAΔ41

Natalie Zeytuni, Raz Zarivach

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Magnetotactic bacteria comprise a diverse group of aquatic microorganisms that are able to orientate themselves along geomagnetic fields. This behavior is believed to aid their search for suitable environments (1). This capability is conferred by the magnetosome, a subcellular organelle that consists of a linear-chain assembly of lipid vesicles each able to biomineralize and enclose a ~50-nm crystal of magnetite or greigite. A principle component of the magnetosome that was shown to be required for the formation of functional vesicles is MamA. MamA is a highly abundant magnetosome-associated protein which is one of the most characterized magnetosome-associated proteins in vivo (2-6). This article focuses on the purification of MamA, which despite being studied in vivo, no clear functional or structural details have been identified for it. Bioinformatics analysis suggested that MamA is a tetra-tricopeptide repeat (TPR) containing protein. TPR is a structural motif found as such or forming part of a bigger fold in a wide range of proteins, it serves as a template for protein-protein interactions and mediates multi-protein complexes (7). TPRs are involved in many crucial tasks in eukaryotic cell organelle processes and many bacterial pathways (8-14). In order to understand MamA, a unique TPR containing protein, highly purified protein is required as a first step. In this article, we present the purification protocol for a stable MamA deletion mutant (MamAΔ41) from M. magneticum AMB-1.

Original languageEnglish
Article numbere1844
JournalJournal of Visualized Experiments
Issue number37
DOIs
StatePublished - 1 Jan 2010

Keywords

  • Cellular biology
  • Issue 37
  • Magnetosome
  • Magnetotactic bacteria
  • MamA
  • Recombinant protein purification

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