Quantitative determinations of microcytic-hypochromic red blood cell population and glycerol permeability in iron-deficiency anemia and β thalassemia minor

T. Yermiahu, M. Ben-Shalom, A. Porath, H. Vardi, A. Boantza, D. Mazor, N. Meyerstein

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The H1/H2 Technicon automated cell analyzer measures, in addition to the usual red blood cell (RBC) parameters, subpopulations of microcytic (M) and hypochromic (H) red blood cells. The M/H ratio may be useful in the differential diagnosis of iron-deficiency anemia (IDA) and β thalassemia minor (Thal). Thirty-three iron-deficient patients and 26 thalassemia patients were studied. The M/H ratio was found to be higher in thalassemia patients than in IDA patients. Using a cut-off point of 1.9 M/H ratio, the calculated discriminant efficiency was 88%. When glycerol lysis values were determined at 70 s as a cut-off point, the discriminant efficiency was slightly higher, at 91%. Thus, the combination of the M/H ratio and the glycerol lysis time (GLT) improves the discriminant efficiency and provides a good diagnostic tool to differentiate between the two microcytic-hypochromic anemias. The study suggests that the M/H ratio together with the GLT could serve as a useful screening tool, prior to the application of other more sophisticated methods.

Original languageEnglish
Pages (from-to)468-471
Number of pages4
JournalAnnals of Hematology
Volume78
Issue number10
DOIs
StatePublished - 1 Oct 1999

Keywords

  • Glycerol lysis time
  • H1/H2 Technicon autoanalyzer
  • M/H ratio

ASJC Scopus subject areas

  • Hematology

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