Quantitative phase microscopy of articular chondrocyte dynamics by wide-field digital interferometry

Natan T. Shaked, John D. Finan, Farshid Guilak, Adam Wax

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

We experimentally implement label-free phase microscopy using wide-field digital interferometry (WFDI) techniques to retrieve quantitative volumetric data of articular chondrocyte dynamics. Using the scanless interferometric system, we visualize chondrocyte swelling and bursting induced by hypo-osmotic pressure. Reconstructed images are obtained by an efficient digital process. We use the resulting images to calculate quantitative temporal-spatial morphological parameters of the cell, with the observed dynamics limited only by the true frame rate of the camera. To show the utility of WFDI in recording articular chondrocyte dynamics, we also provide an experimental comparison of WFDI and differential interference contrast microscopy.

Original languageEnglish
Article number010505
JournalJournal of Biomedical Optics
Volume15
Issue number1
DOIs
StatePublished - 1 Dec 2010
Externally publishedYes

Keywords

  • Cell imaging
  • Chondrocytes
  • Cytology
  • Interferometric microscopy
  • Osmotic stress
  • Phase microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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