Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States

Sarah M. Allard; Sultana Solaiman; Mary Theresa Callahan; Anthony Bui; Hillary Craddock; Joseph Haymaker; Derek Foust; Rico Duncan; Eoghan Smyth; Emmanuel F. Mongodin; Fawzy Hashem; Eric May; Shirley A. Micallef; Amy R. Sapkota

doi:10.1016/j.envres.2019.02.022

Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States

Sarah M. Allard, Sultana Solaiman, Mary Theresa Callahan, Anthony Bui, Hillary Craddock, Joseph Haymaker, Derek Foust, Rico Duncan, Eoghan Smyth, Emmanuel F. Mongodin, Fawzy Hashem, Eric May, Shirley A. Micallef, Amy R. Sapkota

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.

Original language	English
Pages (from-to)	296-300
Number of pages	5
Journal	Environmental Research
Volume	172
DOIs	https://doi.org/10.1016/j.envres.2019.02.022
State	Published - 1 May 2019
Externally published	Yes

Keywords

16S rRNA
Quenching
Reclaimed water
Sodium thiosulfate
Water microbiome

ASJC Scopus subject areas

Biochemistry
General Environmental Science

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10.1016/j.envres.2019.02.022

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Allard, S. M., Solaiman, S., Callahan, M. T., Bui, A., Craddock, H., Haymaker, J., Foust, D., Duncan, R., Smyth, E., Mongodin, E. F., Hashem, F., May, E., Micallef, S. A., & Sapkota, A. R. (2019). Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States. Environmental Research, 172, 296-300. https://doi.org/10.1016/j.envres.2019.02.022

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title = "Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States",

abstract = "A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.",

keywords = "16S rRNA, Quenching, Reclaimed water, Sodium thiosulfate, Water microbiome",

author = "Allard, {Sarah M.} and Sultana Solaiman and Callahan, {Mary Theresa} and Anthony Bui and Hillary Craddock and Joseph Haymaker and Derek Foust and Rico Duncan and Eoghan Smyth and Mongodin, {Emmanuel F.} and Fawzy Hashem and Eric May and Micallef, {Shirley A.} and Sapkota, {Amy R.}",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

month = may,

day = "1",

doi = "10.1016/j.envres.2019.02.022",

language = "English",

volume = "172",

pages = "296--300",

journal = "Environmental Research",

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publisher = "Academic Press Inc.",

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Allard, SM, Solaiman, S, Callahan, MT, Bui, A, Craddock, H, Haymaker, J, Foust, D, Duncan, R, Smyth, E, Mongodin, EF, Hashem, F, May, E, Micallef, SA & Sapkota, AR 2019, 'Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States', Environmental Research, vol. 172, pp. 296-300. https://doi.org/10.1016/j.envres.2019.02.022

TY - JOUR

T1 - Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States

AU - Allard, Sarah M.

AU - Solaiman, Sultana

AU - Callahan, Mary Theresa

AU - Bui, Anthony

AU - Craddock, Hillary

AU - Haymaker, Joseph

AU - Foust, Derek

AU - Duncan, Rico

AU - Smyth, Eoghan

AU - Mongodin, Emmanuel F.

AU - Hashem, Fawzy

AU - May, Eric

AU - Micallef, Shirley A.

AU - Sapkota, Amy R.

PY - 2019/5/1

Y1 - 2019/5/1

N2 - A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.

AB - A quenching agent is commonly added to chlorinated, reclaimed water during sample collection to prevent chlorine-mediated die-off of viable microbiota. However, the effect of quenching on downstream 16S rRNA-based bacterial community analyses is unclear. We conducted a side-by-side comparison of 16S rRNA sequencing data from reclaimed water samples quenched with sodium thiosulfate and non-quenched samples. Our data showed that 16 S rRNA processing and sequencing methods, and resulting bacterial profiles, were not negatively impacted by quenching.

KW - 16S rRNA

KW - Quenching

KW - Reclaimed water

KW - Sodium thiosulfate

KW - Water microbiome

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U2 - 10.1016/j.envres.2019.02.022

DO - 10.1016/j.envres.2019.02.022

M3 - Article

C2 - 30822563

AN - SCOPUS:85061978504

SN - 0013-9351

VL - 172

SP - 296

EP - 300

JO - Environmental Research

JF - Environmental Research

ER -

Quenching by sodium thiosulfate does not influence 16S rRNA gene sequencing profiles of reclaimed water from three sites in the Mid-Atlantic, United States

Abstract

Keywords

ASJC Scopus subject areas

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