Radical scavengers suppress low frequency EMF enhanced proliferation in cultured cells and stress effects in higher plants

Abraham H. Parola, Daniel Kost, Gila Katsir, Edna Ben Izhak Monselise, Rivka Cohen-Luria

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

In previous publications, we reported that sinusoidal varying magnetic fields (SVMF) modify the activity and dynamics of the malignancy marker adenosine deaminase, and enhance the proliferation of chick embryo fibroblasts (CEF). While the SVMF examined by us (50, 60 & 100 Hz / 0.06-0.7 mT) were all below kT, they may have the potential of altering chemical processes in which excited radicals are involved. We tested this hypothesis in two experimental systems: CEF in culture and Spirodela oligorrhiza (Lemnaceae) (a small aquatic plant, commonly known as Duckweed). CEF were exposed to SVMF of 100 Hz/0.7 mT for 24 h. The addition of the exogenous radical scavengers catalase, superoxide dismutase or vitamin E to the cells during exposure significantly suppressed enhancement of cell proliferation caused by the field (by 79, 67 and 82%, respectively, as evaluated by the MTT colorimetric assay). 15N NMR analysis of Duckweed plants fed by 15N-labeled ammonium chloride and exposed to SVMF at 60 and 100 Hz/0.7 mT for 24 h, revealed augmented alanine production. Alanine did not accumulate in the absence of SVMF. The addition of vitamin C, a radical scavenger, reduced alanine production by 82%. Exposure to SVMF resulted in specific metabolic stress effects in Duckweed plants and enhanced proliferation of CEF. In both cases, it is suggested that free radicals are involved.

Original languageEnglish
Pages (from-to)103-111
Number of pages9
JournalEnvironmentalist
Volume25
Issue number2-4 SPEC. ISS.
DOIs
StatePublished - 1 Dec 2005

Keywords

  • Alanine production
  • CEF
  • Catalase
  • Etiolated plants
  • Free radicals
  • MTT
  • N NMR
  • Nitrogen assimilation
  • SVMF
  • Spirodela oligorrhiza (Lemnaceae) (Duckweed)
  • Superoxide dismutase
  • Vitamin C (ascorbic acid)
  • Vitamin E (α-tocopherol)

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