Rapid optimization of labeled ubiquitinated peptides for monitoring deubiquitinases activities

Shimrit Ohayon; Maya Refua; Ashraf Brik

doi:10.1039/c5ob01142f

Rapid optimization of labeled ubiquitinated peptides for monitoring deubiquitinases activities

Shimrit Ohayon
, Maya Refua
, Ashraf Brik

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Studying and targeting deubiquitinases is of high importance due to their various roles in cellular processes and involvement in diseases such as cancer. The recent development of unique probes and reagents using chemical synthesis of proteins became very instrumental in supporting these efforts. Here, we present a protein synthetic approach that enables the rapid synthesis of differently modified labeled-ubiquitinated peptides to facilitate rapid optimization of deubiquitinase substrates.

Original language	English
Pages (from-to)	8182-8186
Number of pages	5
Journal	Organic and Biomolecular Chemistry
Volume	13
Issue number	30
DOIs	https://doi.org/10.1039/c5ob01142f
State	Published - 14 Aug 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1039/c5ob01142f

Cite this

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title = "Rapid optimization of labeled ubiquitinated peptides for monitoring deubiquitinases activities",

abstract = "Studying and targeting deubiquitinases is of high importance due to their various roles in cellular processes and involvement in diseases such as cancer. The recent development of unique probes and reagents using chemical synthesis of proteins became very instrumental in supporting these efforts. Here, we present a protein synthetic approach that enables the rapid synthesis of differently modified labeled-ubiquitinated peptides to facilitate rapid optimization of deubiquitinase substrates.",

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AU - Ohayon, Shimrit

AU - Refua, Maya

AU - Brik, Ashraf

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AB - Studying and targeting deubiquitinases is of high importance due to their various roles in cellular processes and involvement in diseases such as cancer. The recent development of unique probes and reagents using chemical synthesis of proteins became very instrumental in supporting these efforts. Here, we present a protein synthetic approach that enables the rapid synthesis of differently modified labeled-ubiquitinated peptides to facilitate rapid optimization of deubiquitinase substrates.

UR - https://www.scopus.com/pages/publications/84937718672

U2 - 10.1039/c5ob01142f

DO - 10.1039/c5ob01142f

M3 - Article

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AN - SCOPUS:84937718672

SN - 1477-0520

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JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

IS - 30

ER -

Rapid optimization of labeled ubiquitinated peptides for monitoring deubiquitinases activities

Abstract

UN SDGs

ASJC Scopus subject areas

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