Rat epiphyseal cells in culture: Responsiveness to bone-seeking hormones

J. Levy; Z. Shimshoni; D. Somjen; E. Berger; N. Fine; M. Silbermann; I. Binderman

doi:10.1007/BF02623598

Rat epiphyseal cells in culture: Responsiveness to bone-seeking hormones

J. Levy
, Z. Shimshoni
, D. Somjen
, E. Berger
, N. Fine
, M. Silbermann
, I. Binderman

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJ_b medium supplemented with 10% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50% within 15 min of treatment, whereas prostaglandin E₂ (PGE₂) caused an increase of 160%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE₂ (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)₂D₃ increased significantly the [³H]thymidine incorporation into DNA, whereas 1,25(OH)₂D₃ effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness.

Original language	English
Pages (from-to)	620-624
Number of pages	5
Journal	In Vitro Cellular & Developmental Biology
Volume	24
Issue number	7
DOIs	https://doi.org/10.1007/BF02623598
State	Published - 1 Jul 1988
Externally published	Yes

Keywords

DNA
cAMP
calcitonin
epiphyseal cartilage cells
parathyroid hormone
prostaglandin E
vitamin D metabolites

ASJC Scopus subject areas

Developmental Biology
Clinical Biochemistry
Cell Biology

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10.1007/BF02623598

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title = "Rat epiphyseal cells in culture: Responsiveness to bone-seeking hormones",

abstract = "Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJb medium supplemented with 10\% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50\% within 15 min of treatment, whereas prostaglandin E2 (PGE2) caused an increase of 160\%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE2 (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)2D3 increased significantly the [3H]thymidine incorporation into DNA, whereas 1,25(OH)2D3 effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness.",

keywords = "DNA, cAMP, calcitonin, epiphyseal cartilage cells, parathyroid hormone, prostaglandin E, vitamin D metabolites",

author = "J. Levy and Z. Shimshoni and D. Somjen and E. Berger and N. Fine and M. Silbermann and I. Binderman",

year = "1988",

month = jul,

day = "1",

doi = "10.1007/BF02623598",

language = "English",

volume = "24",

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T2 - Responsiveness to bone-seeking hormones

AU - Levy, J.

AU - Shimshoni, Z.

AU - Somjen, D.

AU - Berger, E.

AU - Fine, N.

AU - Silbermann, M.

AU - Binderman, I.

PY - 1988/7/1

Y1 - 1988/7/1

N2 - Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJb medium supplemented with 10% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50% within 15 min of treatment, whereas prostaglandin E2 (PGE2) caused an increase of 160%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE2 (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)2D3 increased significantly the [3H]thymidine incorporation into DNA, whereas 1,25(OH)2D3 effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness.

AB - Cell cultures derived from young rat epiphyseal cartilage were grown for approximately 2 wk in BGJb medium supplemented with 10% fetal bovine serum to reach confluence. These cells were identified as chondrocytes as checked by morphology, the presence of alkaline phosphatase, and a positive type II collagen antibody reaction. The cells also responded to different hormonal treatment. Parathyroid hormone (PTH) increased cyclic AMP production by 50% within 15 min of treatment, whereas prostaglandin E2 (PGE2) caused an increase of 160%. Calcitonin (CT) did not affect cAMP production in these cells. DNA synthesis 24 h after hormonal treatment was increased by PTH (2.5-fold) and PGE2 (2-fold), but not by CT. Among the vitamin D metabolites, 24,25(OH)2D3 increased significantly the [3H]thymidine incorporation into DNA, whereas 1,25(OH)2D3 effect was minimal. These results provide evidence for the use of these cell cultures as a model for cartilage in vitro when studying biological and hormonal responsiveness.

KW - DNA

KW - cAMP

KW - calcitonin

KW - epiphyseal cartilage cells

KW - parathyroid hormone

KW - prostaglandin E

KW - vitamin D metabolites

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M3 - Article

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SN - 0883-8364

VL - 24

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JO - In Vitro Cellular & Developmental Biology

JF - In Vitro Cellular & Developmental Biology

IS - 7

ER -

Rat epiphyseal cells in culture: Responsiveness to bone-seeking hormones

Abstract

Keywords

ASJC Scopus subject areas

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