Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

Shai E. Elizur, Oshrit Lebovitz, Raoul Orvieto, Jehoshua Dor, Tidhar Zan-Bar

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Results: Group A consists of seven women with whom each of the two sibling separate follicle yielded an oocyte that was later discordantly developed to a low- and top-quality embryo. Group B consists of 13 patients in whom one of the sibling follicle yielded an oocyte while the other did not (empty follicle). High-quality embryos were derived from follicles with lower H2O2 levels compared to follicles from which poor-quality embryos developed (1.004 units±0.260 versus 1.145 units±0.236, p<0.02). H2O2 levels were significantly higher (0.951 units±0.233 versus 0.623 units±0.309, p<0.001) in sibling follicles containing oocyte compared to empty follicles.

Conclusion: During the process of ovarian ageing, there might be a gradual increase in H2O2 level in the follicle. Finally, when the follicle ages and becomes empty of oocyte H2O2 levels drops significantly. Therefore, H2O2 levels in FF may serve as a possible marker to determine ovarian aging and follicular metabolic age.

Introduction: The aim of this study was to evaluate the role of oxidative stress in the process of ovarian aging.

Methods: Follicular fluid (FF) from two randomly selected sibling follicles was collected from women undergoing in-vitro fertilization and tested for hydrogen peroxide (H2O2) levels.

Original languageEnglish
Pages (from-to)705-707
Number of pages3
JournalGynecological Endocrinology
Volume30
Issue number10
DOIs
StatePublished - 22 Sep 2014
Externally publishedYes

Keywords

  • Ageing
  • Follicle
  • H2O2
  • Ovary
  • Reactive oxygen species

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Obstetrics and Gynecology

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