Receptor protein tyrosine phosphatase sigma (RPTP-r) increases pro-MMP activity in a trabecular meshwork cell line following oxidative stress conditions

Michal Zaiden, Elie Beit-Yannai

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

PURPOSE. To elucidate the role of phosphatases in the eye drainage system by overexpressing the receptor tyrosine phosphatase sigma (RPTP-σ) in a human normal trabecular meshwork (NTM) cell line. METHODS. The efficacy, expression, and location of RPTP- σ were evaluated following its transfection in NTM cells (NTMT) and in NTM control cells. The cells were also analyzed for viability, matrix metalloproteinase (MMP) activity, and phosphatase activity following oxidative stress conditions. Assays were conducted in the presence or absence of a specific RPTP-σ inhibitor. RESULTS. Transfection efficacy measurements revealed that RPTP-σ expression measured via GFP fluorescence was significantly higher (×3.8) in NTMT cells than in control cells. Western blot analyses showed that RPTP- σ expression was significantly higher (×2.25) in NTMT cells than in control cells. No significant differences were observed in cell viability between NTMT and control cells after oxidative stress. We found that pro-MMP-2 and pro-MMP-9 showed a significantly higher activity (×2.18 and ×1.9; respectively) in NTMT cells than in control cells. Serine/threonine phosphatase activity in NTMT cells was significantly increased following oxidative stress. The specific phosphatase inhibitor PTP-IV inhibited 15% of the RPTP-σ expression in NTM cells and 31% in NTMT cells. The activity of pro-MMP-9, pro- MMP-2, and MMP-9 was significantly inhibited (48%, 35%, and 78% respectively). CONCLUSIONS. The findings indicate that RPTP-σ is expressed constituently in NTM cells and that oxidative stress changes the general phosphatase balance in NTM cells. In addition, the results show that expression levels of RPTP-σ affect the activity of various forms of MMP.

Original languageEnglish
Pages (from-to)5720-5730
Number of pages11
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number10
DOIs
StatePublished - 1 Jan 2015

Keywords

  • Glaucoma
  • MMPs
  • Oxidative stress
  • Phosphatase
  • Trabecular meshwork

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