Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores

Nurit Ashkenasy; Jorge Sánchez-Quesada; Hagan Bayley; M. Reza Ghadiri

doi:10.1002/anie.200462114

Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores

Nurit Ashkenasy, Jorge Sánchez-Quesada, Hagan Bayley, M. Reza Ghadiri

Research output: Contribution to journal › Article › peer-review

172 Scopus citations

Abstract

The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL·DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.

Original language	English
Pages (from-to)	1401-1404
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	9
DOIs	https://doi.org/10.1002/anie.200462114
State	Published - 18 Feb 2005
Externally published	Yes

Keywords

DNA
Host-guest chemistry
Molecular recognition
Rotaxanes
Single-molecule studies

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200462114

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abstract = "The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL·DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.",

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AU - Sánchez-Quesada, Jorge

AU - Bayley, Hagan

AU - Ghadiri, M. Reza

PY - 2005/2/18

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AB - The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL·DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.

KW - DNA

KW - Host-guest chemistry

KW - Molecular recognition

KW - Rotaxanes

KW - Single-molecule studies

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DO - 10.1002/anie.200462114

M3 - Article

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Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores

Abstract

Keywords

ASJC Scopus subject areas

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