Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores

Nurit Ashkenasy, Jorge Sánchez-Quesada, Hagan Bayley, M. Reza Ghadiri

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL·DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA.

Original languageEnglish
Pages (from-to)1401-1404
Number of pages4
JournalAngewandte Chemie - International Edition
Volume44
Issue number9
DOIs
StatePublished - 18 Feb 2005
Externally publishedYes

Keywords

  • DNA
  • Host-guest chemistry
  • Molecular recognition
  • Rotaxanes
  • Single-molecule studies

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry

Fingerprint

Dive into the research topics of 'Recognizing a single base in an individual DNA strand: A step toward DNA sequencing in nanopores'. Together they form a unique fingerprint.

Cite this