Reconfiguring primase DNA-recognition sequences by using a data driven approach

Adam Soffer, Morya Ifrach, Stefan Ilic, Ariel Afek, Dan Vilenchik, Barak Akabayov

Research output: Working paper/PreprintPreprint

Abstract

DNA–protein interactions are essential in all aspects of every living cell. Understanding of how features embedded in the DNA sequence affect specific interactions with proteins is challenging but important, since it may contribute to finding the means to regulate metabolic pathways involving DNA–protein interactions. Using a massive experimental benchmark dataset of binding scores for DNA sequences and a machine learning workflow, we describe the binding to DNA of T7 primase, as a model system for specific DNA–protein interactions. Effective binding of T7 primase to its specific DNA recognition se-quences triggers the formation of RNA primers that serve as Okazaki fragment start sites during DNA replication.
Original languageEnglish GB
DOIs
StatePublished - 2020

Publication series

NamebioRxiv
PublisherCold Spring Harbor Laboratory Press

Fingerprint

Dive into the research topics of 'Reconfiguring primase DNA-recognition sequences by using a data driven approach'. Together they form a unique fingerprint.

Cite this