Reconstitution of purified VDAC1 into a lipid bilayer and recording of channel conductance.

Danya Ben-Hail, Varda Shoshan-Barmatz

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The functional properties of purified voltage-dependent anion-selective channel protein 1 (VDAC1) have been examined in reconstituted systems based on artificially prepared phospholipid bilayers. The most widespread method for the characterization of the pore-forming activity of the mitochondrial VDAC1 protein requires reconstitution of the channel activity into a planar lipid bilayer (PLB) that separates two aqueous compartments. This system is able to produce a refined and large set of information on channel activity. The activity of the channel is reflected in the flow of ions (i.e., current) through a membrane that otherwise represents a barrier to ion flow. The setup thus requires the use of purified protein and a source of continuous current, as well as a sophisticated detector system able to amplify and record low, picoamper-level currents. This system is so efficient that the activity of even a single channel can be detected, allowing for study of VDAC1 at the molecular level.

Original languageEnglish
Pages (from-to)100-105
Number of pages6
JournalCold Spring Harbor Protocols
Volume2014
Issue number1
DOIs
StatePublished - 1 Jan 2014

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