Red blood cell permeability to thiol compounds following oxidative stress

D. Mazor, E. Golan, V. Philip, M. Katz, A. Jafe, Z. Ben-Zvi, N. Meyerstein

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49 Scopus citations

Abstract

The permeability of red blood cells (RBCs) to thiol containing compounds, reduced glutathione (GSH) and N-acetyl cysteine (NAC), has been studied in control adult and neonatal cells and after oxidative stress. NAC penetrates the cell membrane easily while GSH hardly permeates. We measured their capacity to enhance intracellular non-protein thiols (NPSH), after inducing damage to the membrane by formation of defects. Diamide, phenazine methosulfate (PMS) and t-butyl hydroperoxide (BHP) were chosen as exogenous oxidants, each inducing damage by a different mechanism, Our data indicate that although neonatal cells are more sensitive to oxidative stress, only membrane damage induced by diamide, renders adult and neonatal cells permeable to GSH. NAC treatment enhances thiol levels in cells exposed to oxidizing agents, as well as in control cells.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalEuropean Journal of Haematology
Volume57
Issue number3
DOIs
StatePublished - 1 Jan 1996

Keywords

  • Glutathione
  • NAC
  • Oxidative stress
  • Red blood cells

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