Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability

Aviv Rom, Liliya Melamed, Noa Gil, Micah Jonathan Goldrich, Rotem Kadir, Matan Golan, Inbal Biton, Rotem Ben Tov Perry, Igor Ulitsky

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Chromodomain helicase DNA binding protein 2 (Chd2) is a chromatin remodeller implicated in neurological disease. Here we show that Chaserr, a highly conserved long noncoding RNA transcribed from a region near the transcription start site of Chd2 and on the same strand, acts in concert with the CHD2 protein to maintain proper Chd2 expression levels. Loss of Chaserr in mice leads to early postnatal lethality in homozygous mice, and severe growth retardation in heterozygotes. Mechanistically, loss of Chaserr leads to substantially increased Chd2 mRNA and protein levels, which in turn lead to transcriptional interference by inhibiting promoters found downstream of highly expressed genes. We further show that Chaserr production represses Chd2 expression solely in cis, and that the phenotypic consequences of Chaserr loss are rescued when Chd2 is perturbed as well. Targeting Chaserr is thus a potential strategy for increasing CHD2 levels in haploinsufficient individuals.

Original languageEnglish
Article number5092
JournalNature Communications
Volume10
Issue number1
DOIs
StatePublished - 8 Nov 2019

ASJC Scopus subject areas

  • Chemistry (all)
  • Biochemistry, Genetics and Molecular Biology (all)
  • Physics and Astronomy (all)

Fingerprint

Dive into the research topics of 'Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability'. Together they form a unique fingerprint.

Cite this