TY - JOUR
T1 - Regulation of cyclic AMP level and lactic acid production in Ehrlich ascites tumor cells
AU - Graziani, Yosef
AU - Winikoff, Judy
AU - Chayoth, Reuben
N1 - Funding Information:
The authors wish to thank Professor E. Racker for his valuable suggestions. This work was supported in part by the B. De Rothschild foundation for the advancement of science in Israel.
PY - 1977/4/27
Y1 - 1977/4/27
N2 - 1. 1.Quercetin (3,3′,4′,7-pentahydroxy flavone) at the concentration of 10-4M, as well as 2·10-2 M theophylline and 1.5·10-4 M prostaglandin E2 caused maximal rise of cyclic AMP in Ehrlich ascites tumor cells. 2. 2. No additional increase of cyclic AMP level in these cells was found when both quercetin (10-4 M) and theophylline (2·10-2 M) were present in the incubation medium. while combination of quercetin (10-4 M) and prostaglandin E2 (1.5·10-4 M) has a synergistic effect on the level of cyclic AMP. 3. 3. Degradation of cyclic AMP by homogenate pf Ehrlich ascites tumor cells was inhibited by both quercetin and theophylline. 4. 4. Quercetin, and to a smaller but significant extent theophylline, inhibited the lactic acid production in Ehrlich ascites tumor cells while prostaglandin E2 did not change the glycolytic rate in these cells. No synergistic inhibitory effect on lactic acid production was found when combinations of quercetin and prostaglandin E2, quercetin and theophylline or prostaglandin E2 and theophylline were tested. 5. 5. Treatment of Ehrlich ascites tumor cells with dextran sulfate abolished the inhibitory effect of quercetin on lactic acid production, while the effect of the bioflavonoid on cyclic AMP levels was not altered.
AB - 1. 1.Quercetin (3,3′,4′,7-pentahydroxy flavone) at the concentration of 10-4M, as well as 2·10-2 M theophylline and 1.5·10-4 M prostaglandin E2 caused maximal rise of cyclic AMP in Ehrlich ascites tumor cells. 2. 2. No additional increase of cyclic AMP level in these cells was found when both quercetin (10-4 M) and theophylline (2·10-2 M) were present in the incubation medium. while combination of quercetin (10-4 M) and prostaglandin E2 (1.5·10-4 M) has a synergistic effect on the level of cyclic AMP. 3. 3. Degradation of cyclic AMP by homogenate pf Ehrlich ascites tumor cells was inhibited by both quercetin and theophylline. 4. 4. Quercetin, and to a smaller but significant extent theophylline, inhibited the lactic acid production in Ehrlich ascites tumor cells while prostaglandin E2 did not change the glycolytic rate in these cells. No synergistic inhibitory effect on lactic acid production was found when combinations of quercetin and prostaglandin E2, quercetin and theophylline or prostaglandin E2 and theophylline were tested. 5. 5. Treatment of Ehrlich ascites tumor cells with dextran sulfate abolished the inhibitory effect of quercetin on lactic acid production, while the effect of the bioflavonoid on cyclic AMP levels was not altered.
UR - http://www.scopus.com/inward/record.url?scp=0017575467&partnerID=8YFLogxK
U2 - 10.1016/0304-4165(77)90207-0
DO - 10.1016/0304-4165(77)90207-0
M3 - Article
AN - SCOPUS:0017575467
SN - 0304-4165
VL - 497
SP - 499
EP - 506
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
IS - 2
ER -