Regulation of cyclic amp level and synthesis of DNA, RNA and protein by quercetin in ehrlich ascites tumor cells

Y. Graziani, R. Chayoth

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Abstract

Quercetin (3.3, 4′, 5.7-pentahydroxy flavone) at the concentration of 10-4 M as well as 10-2 M theophylline and 10-3 M dibutyryl cyclic AMP caused at least 85 per cent inhibition of [3H]thymidine incorporation in Ehrlich ascites tumor cells. At the same concentrations, these drugs decreased [3H]uridine and [3H]-L-leucine incorporation by 50-60 per cent and 35-45 per cent respectively. Ouabain (10-3 M), the specific inhibitor of Na+-K+ pump system, did not alter the incorporation of [3H]thymidine and [3]uridine, but decreased the incorporation of [3H]-L-leucine in these cells. Treatment of Ehrlich ascites tumor cells with the polyanion dextran sulfate did not change the inhibitory effect of quercetin, theophylline and dibutyrlyl cyclic AMP on [3H]thymidine incorporation. On the other hand, this polyanion decreased the inhibitory effect of these drugs on incorporation of [3H]uridine and abolished completely their effect on incorporation of [3H]-L-leucine.

Original languageEnglish
Pages (from-to)397-403
Number of pages7
JournalBiochemical Pharmacology
Volume28
Issue number3
DOIs
StatePublished - 1 Jan 1979

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