Regulation of lipopolysaccharide-induced granulopoiesis and macrophage formation by spleen cells. II. Macrophage-lymphocyte interactions in the process of generation of colony-stimulating factor

R. N. Apte, F. Hertogs Ch., D. H. Pluznik

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Addition of bacterial lipopolysaccharide (LPS), a B cell mitogen, to mouse spleen cell cultures strongly stimulated production of colony-stimulating factor (CSF), the humoral regulator of granulopoiesis and macrophage formation in vitro. Splenic-purified lymphocyte generated only small amounts of CSF in response to LPS; however, when a critical number of proteose peptone-stimulated macrophages (about 5 to 10%) was added to the cultures, the ability to secrete LPS-induced CSF was restored. CSF generation in the macrophage-lymphocyte mixed cultures was found to be mediated via a uni-directional pathway whereby LPS-stimulated macrophages secreted lymphostimulatory diffusible factor(s), which replaced intact cells in the cooperative events with lymphocytes. Macrophage potent supernatants activated lymphocytes from congenitally athymic nude mice that, in turn, secreted CSF, indicating that this granulopoietic mediator is probably a lymphokine of B cell origin. The synthetic compound 2-mercaptoethanol could not act as a sufficient substitute for macrophages in generation of CSF in vitro, further pointing to the specific accessory role of macrophages in this secretory process. Macrophages from LPS low responder mice of the strain C3H/HeJ failed to elaborate this lymphostimulatory activity; however, lymphocytes from these mice could be activated to secrete CSF by potent macrophage-derived culture fluids, although to a lesser extent than lymphocytes from LPS high responder mice.

Original languageEnglish
Pages (from-to)1223-1229
Number of pages7
JournalJournal of Immunology
Volume124
Issue number3
StatePublished - 1 Jan 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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