Regulation of T cell receptor γ gene expression by cytokines in murine hematopoietic cells

Y. Weinstein, D. S. Askew, O. Miura, J. L. Cleveland, J. N. Ihle

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Murine IL-3-dependent myeloid cell lines express transcripts from non-rearranged TCRγ genes and this expression is dependent upon IL-3. To investigate this observation in general terms we examined various IL-3 dependent cell lines for TCRγ gene expression. We also examined various cytokines to test their potential to induce TCRγ gene expression. All IL-3 dependent cell lines expressed TCRγ transcripts. The IL-3 induced expression was sensitive to protein synthesis inhibitors. This demonstrated that the TCRγ genes belong to the early growth factor response class. IL-3, IL-4, GM-CSF and Erytropoietin (EPO), but not G-CSF, induced TCR gene expression. 32D cells transfected with the IL-2β chain receptor became responsive to IL-2 as a growth factor and induced TCRγ gamma gene expression. The induction of TCRγ gene expression by the cytokines was not correlated to their growth promoting activity. This indicated different signaling pathways.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalEuropean Cytokine Network
Issue number2
StatePublished - 1 Jan 1995


  • EPO
  • IL-2
  • IL-3
  • IL-4
  • Signal transduction
  • T cell receptor gamma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry


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