Abstract
We developed a method to identify the insertion sites of transposons in the chromosome of Salmonella using one step only. In this method, the Salmonella's genomic DNA is directly sequenced using a transposon internal primer. Reliable direct sequencing was achieved using high purity genomic DNA and an improved protocol for automated sequence machine. This note is intended to promote the use of direct sequencing, which we found to be reliable, efficient and inexpensive as compared to the other currently used methods.
Original language | English |
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Pages (from-to) | 137-140 |
Number of pages | 4 |
Journal | Journal of Microbiological Methods |
Volume | 54 |
Issue number | 1 |
DOIs | |
State | Published - 1 Jul 2003 |
Keywords
- Direct genomic sequencing
- Transposon
- Transposon insertion site
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Microbiology (medical)