Requirement for the acetyl phosphate pathway in Escherichia coli ATP-dependent proteolysis

Itzhak Mizrahi, Dvora Biran, Eliora Z. Ron

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Protein degradation is a central component of the protein quality control system. Here we show that efficient proteolysis in Escherichia coli requires the active acetyl phosphate pathway. Deletion of this pathway, leading to depletion of acetyl phosphate, results in temperature sensitivity and reduced rate of ATP-dependent proteolysis. The effect on proteolysis is general, as can be seen from the slowing down of the degradation of unstable proteins, including puromycin-derived peptides. In addition, reduced intracellular concentrations of acetyl phosphate brings about an increase in the levels of protein aggregates, which contain a wide range of proteins, as expected if a broad spectrum of substrates are involved. Additional outcomes of acetyl phosphate deficiency are elevation in the transcript levels of heat shock genes and increased thermotolerance. In E. coli the acetyl phosphate pathway is the only source of acetyl phosphate, which is a key metabolic compound involved in major cellular processes. In this communication we present evidence for the general role of the acetyl phosphate pathway in protein degradation.

Original languageEnglish
Pages (from-to)201-211
Number of pages11
JournalMolecular Microbiology
Issue number1
StatePublished - 1 Oct 2006
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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