Abstract
The ESCRT machinery mediates membrane fission in a variety of processes in cells. According to current models, ESCRT-III proteins drive membrane fission by assembling into helical filaments on membranes. Here, we used 3D STORM imaging of endogenous ESCRT-III component IST1 to reveal the evolution of the structural organization of ESCRT-III in mammalian cytokinetic abscission. Using this approach, ESCRT-III ring and spiral assemblies were resolved and characterized at different stages of abscission. Visualization of IST1 structures in cells lacking the microtubule-severing enzyme spastin and in cells depleted of specific ESCRT-III components or the ATPase VPS4 demonstrated the contribution of these components to the organization and function of ESCRTs in cells. This work provides direct evidence that ESCRT-III proteins form helical filaments to mediate their function in cells and raises new mechanistic scenarios for ESCRT-driven cytokinetic abscission. The ESCRT complex drives membrane constriction and fission in a variety of process in cells. In this work, Goliand et al. used 3D STORM imaging to resolve the structure of the ESCRT-III complex during abscission of the intercellular bridge connecting two dividing cells, highlighting mechanistic steps in ESCRT cellular function.
Original language | English |
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Pages (from-to) | 1756-1764 |
Number of pages | 9 |
Journal | Cell Reports |
Volume | 24 |
Issue number | 7 |
DOIs | |
State | Published - 14 Aug 2018 |
Keywords
- ESCRT machinery
- abscission
- cytokinesis
- membrane fission
- super resolution microscopy
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology