TY - JOUR
T1 - Responses of mesenchymal stem cell to chitosan-coralline composites microstructured using coralline as gas forming agent
AU - Gravel, Mylène
AU - Gross, Talia
AU - Vago, Razi
AU - Tabrizian, Maryam
N1 - Funding Information:
This work was funded by Ministère du Développement Économique et Régional et de la Recherche (MDERR) Fonds Québécois de Recherche sur la Nature et les Technologies (FQRNT)-regroupment stratégique, and by the Natural Sciences and Engineering Research Council of Canada (NSERC).
PY - 2006/3/1
Y1 - 2006/3/1
N2 - Macroporous composites made of coralline:chitosan with new microstructural features were studied for their scaffolding potential in in vitro bone regeneration. By using different ratios of natural coralline powder, as in situ gas forming agent and reinforcing phase, followed by freeze-drying, scaffolds with controlled porosity and pore structure were prepared and cultured with mesenchymal stem cells (MSCs). Their supportive activity of cellular attachment, proliferation and differentiation were assessed through cell morphology studies, DNA content, alkaline phosphatase (ALP) activity and osteocalcin (OC) release. The coralline scaffolds showed by far the highest evaluation of cell number and ALP activity over all the other chitosan-based scaffolds. They were the only material on which the OC protein was released throughout the study. When used as a component of the chitosan composite scaffolds, these coralline's favourable properties seemed to improve the overall performance of the chitosan. Distinct cell morphology and osteoblastic phenotype expression were observed depending on the coralline-to-chitosan ratios composing the scaffolds. The coralline-chitosan composite scaffolds containing high coralline ratios generally showed higher total cell number, ALP activity and OC protein expression comparing to chitosan scaffolds. The results of this study strongly suggest that coralline:chitosan composite, especially those having a high coralline content, may enhance adhesion, proliferation and osteogenic differentiation of MSCs in comparison with pure chitosan. Coralline:chitosan composites could therefore be used as attractive scaffolds for developing new strategies for in vitro tissue engineering.
AB - Macroporous composites made of coralline:chitosan with new microstructural features were studied for their scaffolding potential in in vitro bone regeneration. By using different ratios of natural coralline powder, as in situ gas forming agent and reinforcing phase, followed by freeze-drying, scaffolds with controlled porosity and pore structure were prepared and cultured with mesenchymal stem cells (MSCs). Their supportive activity of cellular attachment, proliferation and differentiation were assessed through cell morphology studies, DNA content, alkaline phosphatase (ALP) activity and osteocalcin (OC) release. The coralline scaffolds showed by far the highest evaluation of cell number and ALP activity over all the other chitosan-based scaffolds. They were the only material on which the OC protein was released throughout the study. When used as a component of the chitosan composite scaffolds, these coralline's favourable properties seemed to improve the overall performance of the chitosan. Distinct cell morphology and osteoblastic phenotype expression were observed depending on the coralline-to-chitosan ratios composing the scaffolds. The coralline-chitosan composite scaffolds containing high coralline ratios generally showed higher total cell number, ALP activity and OC protein expression comparing to chitosan scaffolds. The results of this study strongly suggest that coralline:chitosan composite, especially those having a high coralline content, may enhance adhesion, proliferation and osteogenic differentiation of MSCs in comparison with pure chitosan. Coralline:chitosan composites could therefore be used as attractive scaffolds for developing new strategies for in vitro tissue engineering.
KW - Cell-biomaterial interaction
KW - Chitosan-Coralline scaffolds
KW - Controlled microstructural feature
KW - In situ gas forming agent
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=28744452599&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2005.10.020
DO - 10.1016/j.biomaterials.2005.10.020
M3 - Article
AN - SCOPUS:28744452599
SN - 0142-9612
VL - 27
SP - 1899
EP - 1906
JO - Biomaterials
JF - Biomaterials
IS - 9
ER -