RGD-presenting peptides in amphiphilic and anionic β-sheet hydrogels for improved interactions with cells

Hodaya Green, Guy Ochbaum, Anna Gitelman-Povimonsky, Ronit Bitton, Hanna Rapaport

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

The interest in developing functional biomaterials based on designed peptides has been increasing in recent years. The amphiphilic and anionic β-sheet peptide Pro-Asp-(Phe-Asp)5-Pro, denoted FD, was previously shown to assemble into a hydrogel that induces adsorption of calcium and phosphate ions and formation of the bone mineral hydroxyapatite. In this study the integrin binding peptide, Arg-Gly-Asp (RGD), was incorporated into the hydrogel to assess its influence on an osteoblast culture. In solutions and in hydrogels FD fibrils dominated the assembly structures for up to 25 mol% FD-RGD incorporation. The cellular density of osteoblasts cultured in hydrogels composed of 25 mol% FD-RGD in FD was higher than that of only FD hydrogel cultures. These results demonstrate that RGD and possibly other cell binding motifs can be combined into amphiphilic and anionic β-sheet hydrogels, using the design principles of FD and FD-RGD systems, to enhance interactions with cells.

Original languageEnglish
Pages (from-to)10072-10080
Number of pages9
JournalRSC Advances
Volume8
Issue number18
DOIs
StatePublished - 1 Jan 2018

ASJC Scopus subject areas

  • Chemistry (all)
  • Chemical Engineering (all)

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