RNA labeling and immobilization for nano-displacement measurement: Probing three-dimensional RNA structure

Barak Akabayov, Arnon Henn, Michael Elbaum, Irit Sagi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

RNA molecules play essential roles in many biological processes, including the storage and transfer of information in the cell. These events are mediated via RNA-protein interactions or by catalytic RNA molecules. It is now recognized that unique RNA folds are associated with biological functions. Therefore, to study the intrinsic structural changes and dynamics which regulate the various functions of RNA, it is necessary to probe its three-dimensional structure in solution. In this respect, using single-molecule methodologies may allow study of native RNA molecules independent of their size and in real time. However, this may require the immobilization of RNA on a surface. Here, we report a novel approach to immobilize RNA on a glass. The procedures involve both chemical and enzymatic modifications of long RNA molecules. In addition, we demonstrate the application of an optical tweezers apparatus to measure the length and, hence, the dynamics of immobilized intact ribosomal RNA molecules as a function of different solution conditions.

Original languageEnglish
Pages (from-to)70-74
Number of pages5
JournalIEEE Transactions on Nanobioscience
Volume2
Issue number2
DOIs
StatePublished - 1 Jun 2003
Externally publishedYes

Keywords

  • Optical tweezers
  • RNA
  • RNA immobilization
  • RNA labeling
  • Single molecule

ASJC Scopus subject areas

  • Biotechnology
  • Medicine (miscellaneous)
  • Bioengineering
  • Biomedical Engineering
  • Pharmaceutical Science
  • Computer Science Applications
  • Electrical and Electronic Engineering

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