Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Kuntal Dey; Sayed Modinur Rahaman; Tapati Chakraborti; Sajal Chakraborti

doi:10.1016/j.febslet.2013.09.011

Role of phospholemman and the 70 kDa inhibitor protein in regulating Na⁺/K⁺ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

Kuntal Dey, Sayed Modinur Rahaman, Tapati Chakraborti, Sajal Chakraborti

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na⁺/K⁺ ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na⁺/K ⁺ ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na⁺/K⁺ ATPase in an isoform specific manner during treatment of the cells with U46619.

Original language	English
Pages (from-to)	3535-3540
Number of pages	6
Journal	FEBS Letters
Volume	587
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2013.09.011
State	Published - 1 Nov 2013
Externally published	Yes

Keywords

Inhibitor protein
Na/K ATPase
Phospholemman
Protein kinase
TxA
U46619

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.febslet.2013.09.011

Cite this

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title = "Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation",

abstract = "Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na+/K+ ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na+/K + ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na+/K+ ATPase in an isoform specific manner during treatment of the cells with U46619.",

keywords = "Inhibitor protein, Na/K ATPase, Phospholemman, Protein kinase, TxA, U46619",

author = "Kuntal Dey and Rahaman, {Sayed Modinur} and Tapati Chakraborti and Sajal Chakraborti",

note = "Funding Information: Financial assistance from the Council for Scientific and Industrial Research (CSIR), New Delhi is greatly acknowledged. Thanks are due to Prof. Gerry Shaw (Department of Neuroscience, University of Florida, Gainesville, USA) for his encouragement in our research.",

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T1 - Role of phospholemman and the 70 kDa inhibitor protein in regulating Na+/K+ ATPase activity in pulmonary artery smooth muscle cells under U46619 stimulation

AU - Dey, Kuntal

AU - Rahaman, Sayed Modinur

AU - Chakraborti, Tapati

AU - Chakraborti, Sajal

N1 - Funding Information: Financial assistance from the Council for Scientific and Industrial Research (CSIR), New Delhi is greatly acknowledged. Thanks are due to Prof. Gerry Shaw (Department of Neuroscience, University of Florida, Gainesville, USA) for his encouragement in our research.

PY - 2013/11/1

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N2 - Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na+/K+ ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na+/K + ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na+/K+ ATPase in an isoform specific manner during treatment of the cells with U46619.

AB - Treatment of bovine pulmonary smooth muscle cells with U46619 inhibited the Na+/K+ ATPase activity in two parallel pathways: one of which is mediated via glutathionylation of the pump and the other by augmenting the inhibitory activity of the 70 kDa inhibitor protein of Na+/K + ATPase. Although phospholemman deglutathionylates the pump leading to its activation, the inhibitor is responsible for irreversible inhibition of Na+/K+ ATPase in an isoform specific manner during treatment of the cells with U46619.

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KW - Na/K ATPase

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