Role of PKCα-p 38MAPK-G iα axis in NADPH oxidase derived O 2 .- mediated activation of cPLA 2 under U46619 stimulation in pulmonary artery smooth muscle cells

Sajal Chakraborti, Soumitra Roy, Amritlal Mandal, Kuntal Dey, Animesh Chowdhury, Soni Shaikh, Tapati Chakraborti

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A 2 mimetic, U46619 stimulated NADPH oxidase derived O 2 .- level, which subsequently caused marked increase in [Ca 2+] i [17]. Herein, we demonstrated that O 2 .- mediated increase in [Ca 2+] i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p 38MAPK and that subsequently caused G iα phosphorylation leading to stimulation of cPLA 2 activity in the cell membrane.

Original languageEnglish
Pages (from-to)169-180
Number of pages12
JournalArchives of Biochemistry and Biophysics
Volume523
Issue number2
DOIs
StatePublished - 15 Jul 2012
Externally publishedYes

Keywords

  • cPLA
  • NADPH oxidase
  • p MAPK
  • PKC-α
  • Pulmonary artery smooth muscle cells
  • U46619

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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