Abstract
We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A 2 mimetic, U46619 stimulated NADPH oxidase derived O 2 .- level, which subsequently caused marked increase in [Ca 2+] i [17]. Herein, we demonstrated that O 2 .- mediated increase in [Ca 2+] i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p 38MAPK and that subsequently caused G iα phosphorylation leading to stimulation of cPLA 2 activity in the cell membrane.
Original language | English |
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Pages (from-to) | 169-180 |
Number of pages | 12 |
Journal | Archives of Biochemistry and Biophysics |
Volume | 523 |
Issue number | 2 |
DOIs | |
State | Published - 15 Jul 2012 |
Externally published | Yes |
Keywords
- cPLA
- NADPH oxidase
- p MAPK
- PKC-α
- Pulmonary artery smooth muscle cells
- U46619
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology