Role of PKCα-p 38MAPK-G iα axis in NADPH oxidase derived O 2 .- mediated activation of cPLA 2 under U46619 stimulation in pulmonary artery smooth muscle cells

Sajal Chakraborti; Soumitra Roy; Amritlal Mandal; Kuntal Dey; Animesh Chowdhury; Soni Shaikh; Tapati Chakraborti

doi:10.1016/j.abb.2012.04.017

Role of PKCα-p ³⁸MAPK-G _iα axis in NADPH oxidase derived O ₂ ^.- mediated activation of cPLA ₂ under U46619 stimulation in pulmonary artery smooth muscle cells

Sajal Chakraborti, Soumitra Roy, Amritlal Mandal, Kuntal Dey, Animesh Chowdhury, Soni Shaikh, Tapati Chakraborti

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A ₂ mimetic, U46619 stimulated NADPH oxidase derived O ₂ ^.- level, which subsequently caused marked increase in [Ca ²⁺] _i [17]. Herein, we demonstrated that O ₂ ^.- mediated increase in [Ca ²⁺] _i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p ³⁸MAPK and that subsequently caused G _iα phosphorylation leading to stimulation of cPLA ₂ activity in the cell membrane.

Original language	English
Pages (from-to)	169-180
Number of pages	12
Journal	Archives of Biochemistry and Biophysics
Volume	523
Issue number	2
DOIs	https://doi.org/10.1016/j.abb.2012.04.017
State	Published - 15 Jul 2012
Externally published	Yes

Keywords

NADPH oxidase
PKC-α
Pulmonary artery smooth muscle cells
U46619
cPLA
p MAPK

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.abb.2012.04.017

Cite this

@article{d9bbf5c3075440508400e563aec38254,

title = "Role of PKCα-p 38MAPK-G iα axis in NADPH oxidase derived O 2 .- mediated activation of cPLA 2 under U46619 stimulation in pulmonary artery smooth muscle cells",

abstract = "We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A 2 mimetic, U46619 stimulated NADPH oxidase derived O 2 .- level, which subsequently caused marked increase in [Ca 2+] i [17]. Herein, we demonstrated that O 2 .- mediated increase in [Ca 2+] i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p 38MAPK and that subsequently caused G iα phosphorylation leading to stimulation of cPLA 2 activity in the cell membrane.",

keywords = "NADPH oxidase, PKC-α, Pulmonary artery smooth muscle cells, U46619, cPLA, p MAPK",

author = "Sajal Chakraborti and Soumitra Roy and Amritlal Mandal and Kuntal Dey and Animesh Chowdhury and Soni Shaikh and Tapati Chakraborti",

note = "Funding Information: Financial assistance from the Indian Council of Medical Research (ICMR), New Delhi; the Department of Science & Technology (Govt. of India) and the Department of Atomic Energy (Govt. of India) is greatly acknowledged. This article is dedicated to late Prof. Gail H. Gurtner, Department of Medicine, the Johns Hopkins University, Baltimore, Maryland, USA.",

year = "2012",

month = jul,

day = "15",

doi = "10.1016/j.abb.2012.04.017",

language = "English",

volume = "523",

pages = "169--180",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

number = "2",

}

Role of PKCα-p ³⁸MAPK-G _iα axis in NADPH oxidase derived O ₂ ^.- mediated activation of cPLA ₂ under U46619 stimulation in pulmonary artery smooth muscle cells. / Chakraborti, Sajal; Roy, Soumitra; Mandal, Amritlal et al.
In: Archives of Biochemistry and Biophysics, Vol. 523, No. 2, 15.07.2012, p. 169-180.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Role of PKCα-p 38MAPK-G iα axis in NADPH oxidase derived O 2 .- mediated activation of cPLA 2 under U46619 stimulation in pulmonary artery smooth muscle cells

AU - Chakraborti, Sajal

AU - Roy, Soumitra

AU - Mandal, Amritlal

AU - Dey, Kuntal

AU - Chowdhury, Animesh

AU - Shaikh, Soni

AU - Chakraborti, Tapati

N1 - Funding Information: Financial assistance from the Indian Council of Medical Research (ICMR), New Delhi; the Department of Science & Technology (Govt. of India) and the Department of Atomic Energy (Govt. of India) is greatly acknowledged. This article is dedicated to late Prof. Gail H. Gurtner, Department of Medicine, the Johns Hopkins University, Baltimore, Maryland, USA.

PY - 2012/7/15

Y1 - 2012/7/15

N2 - We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A 2 mimetic, U46619 stimulated NADPH oxidase derived O 2 .- level, which subsequently caused marked increase in [Ca 2+] i [17]. Herein, we demonstrated that O 2 .- mediated increase in [Ca 2+] i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p 38MAPK and that subsequently caused G iα phosphorylation leading to stimulation of cPLA 2 activity in the cell membrane.

AB - We have recently reported that treatment of bovine pulmonary artery smooth muscle cells with the thromboxane A 2 mimetic, U46619 stimulated NADPH oxidase derived O 2 .- level, which subsequently caused marked increase in [Ca 2+] i [17]. Herein, we demonstrated that O 2 .- mediated increase in [Ca 2+] i stimulates an aprotinin sensitive proteinase activity, which proteolytically activates PKC-α under U46619 treatment to the cells. The activated PKC-α then phosphorylates p 38MAPK and that subsequently caused G iα phosphorylation leading to stimulation of cPLA 2 activity in the cell membrane.

KW - NADPH oxidase

KW - PKC-α

KW - Pulmonary artery smooth muscle cells

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KW - cPLA

KW - p MAPK

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