S-Linalool synthase activity in developing fruit of the columnar cactus koubo [Cereus peruvianus (L.) Miller]

Yaron Sitrit, Racheli Ninio, Einat Bar, Einav Golan, Olga Larkov, Uzi Ravid, Efraim Lewinsohn

    Research output: Contribution to journalArticlepeer-review

    15 Scopus citations


    Koubo [Cereus peruvianus (L.) Miller, Cactaceae] is a commercially grown columnar cactus that produces an apple-sized, berry-like, edible fruit. The unique aroma of this fruit is largely due to (S)-linalool and linalool derivatives. Cell-free extracts obtained from koubo fruits produced linalool from geranyl diphosphate (GDP) (apparent K m 18 μM) in enzymatic assays containing the divalent ion cofactor Mn 2+ or Mg 2+. The predominant enantiomer produced in vitro was (S)-linalool, with an optical purity of 85%. Incubation of geranyl diphosphate in the presence of 1-10 mM FeSO 4 or FeCl 2, without added enzyme, supported the in vitro spontaneous formation of racemic linalool. The koubo linalool synthase displayed a molecular mass of 53 kDa as determined by gel permeation chromatography. Enzyme activity levels were negligible in green immature fruits and increased with fruit development and during storage, concomitant with the timing of linalool accumulation in fruits.

    Original languageEnglish
    Pages (from-to)1257-1262
    Number of pages6
    JournalPlant Science
    Issue number6
    StatePublished - 1 Dec 2004


    • (S)-Linalool
    • Cactaceae
    • Cereus peruvianus
    • Fruit
    • Koubo
    • Linalool synthase
    • Ripening
    • Volatiles

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Genetics
    • Plant Science


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