Samase of bacteriophage t3 inactivates escherichia coli’s methionine s-adenosyltransferase by forming heteropolymers

Hadas Simon-Baram, Daniel Kleiner, Fannia Shmulevich, Raz Zarivach, Ran Zalk, Huayuan Tang, Feng Ding, Shimon Bershtein

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

S-Adenosylmethionine lyase (SAMase) of bacteriophage T3 degrades the intracellular SAM pools of the host Escherichia coli cells, thereby inactivating a crucial metabolite involved in a plethora of cellular functions, including DNA methylation. SAMase is the first viral protein expressed upon infection, and its activity prevents methylation of the T3 genome. Maintenance of the phage genome in a fully unme-thylated state has a profound effect on the infection strategy. It allows T3 to shift from a lytic infection under normal growth conditions to a transient lysogenic infection under glucose starvation. Using single-particle cryoelectron microscopy (cryo-EM) and biochemical assays, we demonstrate that SAMase performs its function by not only degrading SAM but also by interacting with and efficiently inhibiting the host’s methionine S-adenosyltransferase (MAT), the enzyme that produces SAM. Specifically, SAMase triggers open-ended head-to-tail assembly of E. coli MAT into an unusual linear filamentous structure in which adjacent MAT tetramers are joined by two SAMase dimers. Molecular dynamics simulations together with normal mode analyses suggest that the entrapment of MAT tetramers within filaments leads to an allosteric inhibition of MAT activity due to a shift to low-frequency, high-amplitude active-site-deforming modes. The amplification of uncorrelated motions between active-site residues weakens MAT's substrate binding affinity, providing a possible explanation for the observed loss of function. We propose that the dual function of SAMase as an enzyme that degrades SAM and as an inhibitor of MAT activity has emerged to achieve an efficient depletion of the intracellular SAM pools. IMPORTANCE Self-assembly of enzymes into filamentous structures in response to specific metabolic cues has recently emerged as a widespread strategy of metabolic regulation. In many instances, filamentation of metabolic enzymes occurs in response to starvation and leads to functional inactivation. Here, we report that bacteriophage T3 modulates the metabolism of the host E. coli cells by recruiting a similar strategy: silencing a central metabolic enzyme by subjecting it to phage-mediated polymeriza-tion. This observation points to an intriguing possibility that virus-induced polymerization of the host metabolic enzymes is a common mechanism implemented by viruses to metabolically reprogram and subdue infected cells.

Original languageEnglish
Article numbere01242-21
JournalmBio
Volume12
Issue number4
DOIs
StatePublished - 1 Aug 2021

Keywords

  • Bacteriophage T3
  • Cryo-EM
  • Enzyme filamentation
  • Metabolic regulation
  • Molecular dynamics (MD) simulations
  • SAMase
  • Virus-host interaction

ASJC Scopus subject areas

  • Virology
  • Microbiology

Fingerprint

Dive into the research topics of 'Samase of bacteriophage t3 inactivates escherichia coli’s methionine s-adenosyltransferase by forming heteropolymers'. Together they form a unique fingerprint.

Cite this