Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms

Guy Indebetouw, Yoshitaka Tada, Joseph Rosen, Gary Brooker

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.

Original languageEnglish
Pages (from-to)993-1000
Number of pages8
JournalApplied Optics
Volume46
Issue number6
DOIs
StatePublished - 20 Feb 2007

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