TY - JOUR
T1 - Schrödinger’s cat among insects
T2 - non-invasive Raman spectroscopy to determine live and dead insects in diapause
AU - Nikelshparg, M. I.
AU - Nikelshparg, E. I.
AU - Bratashov, D. N.
AU - Anikin, V. V.
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to EDP Sciences, Springer-Verlag GmbH Germany, part of Springer Nature 2024.
PY - 2024/5/1
Y1 - 2024/5/1
N2 - Diapause is a peculiar sleep of insects in which the animal's motor activity completely ceases. During this period, insects become a good target for parasitoids, freely attacking them with their mobile ovipositors. We found that the parasitic wasp, Eupelmus messene (Hymenoptera, Eupelmidae, Cynipidae), stirs the internal contents of the diapausing host pupa of Aulacidea hieracii (Bouché, 1834) with its long and flexible ovipositor “making a shake” inside the pupa. However, the attacked pupae stay morphologically indistinguishable from healthy diapausing ones for several months. Using non-invasive Raman spectroscopy (RS), we, for the first time, studied the molecular composition of live diapausing and parasitized A. hieracii pupae. As a result of the study, we discovered alterations in the content and distribution of carotenoids, lipids, and proteins in the attacked pupae compared to the live intact diapausing pupae.
AB - Diapause is a peculiar sleep of insects in which the animal's motor activity completely ceases. During this period, insects become a good target for parasitoids, freely attacking them with their mobile ovipositors. We found that the parasitic wasp, Eupelmus messene (Hymenoptera, Eupelmidae, Cynipidae), stirs the internal contents of the diapausing host pupa of Aulacidea hieracii (Bouché, 1834) with its long and flexible ovipositor “making a shake” inside the pupa. However, the attacked pupae stay morphologically indistinguishable from healthy diapausing ones for several months. Using non-invasive Raman spectroscopy (RS), we, for the first time, studied the molecular composition of live diapausing and parasitized A. hieracii pupae. As a result of the study, we discovered alterations in the content and distribution of carotenoids, lipids, and proteins in the attacked pupae compared to the live intact diapausing pupae.
UR - http://www.scopus.com/inward/record.url?scp=85183745016&partnerID=8YFLogxK
U2 - 10.1140/epjs/s11734-024-01089-3
DO - 10.1140/epjs/s11734-024-01089-3
M3 - Article
AN - SCOPUS:85183745016
SN - 1951-6355
VL - 233
SP - 733
EP - 737
JO - European Physical Journal: Special Topics
JF - European Physical Journal: Special Topics
IS - 3
ER -