Scope and limitation of side-chain assisted ligation

Liat Spasser, K. S. Ajish Kumar, Ashraf Brik

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

Original languageEnglish
Pages (from-to)252-255
Number of pages4
JournalJournal of Peptide Science
Issue number4
StatePublished - 1 Apr 2011


  • Aminoacyl transfer
  • Native chemical ligation
  • Peptide ligation
  • Protein synthesis
  • Removable auxiliary

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry


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