Scope and limitation of side-chain assisted ligation

Liat Spasser; K. S. Ajish Kumar; Ashraf Brik

doi:10.1002/psc.1336

Scope and limitation of side-chain assisted ligation

Liat Spasser, K. S. Ajish Kumar, Ashraf Brik

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

Original language	English
Pages (from-to)	252-255
Number of pages	4
Journal	Journal of Peptide Science
Volume	17
Issue number	4
DOIs	https://doi.org/10.1002/psc.1336
State	Published - 1 Apr 2011

Keywords

Aminoacyl transfer
Native chemical ligation
Peptide ligation
Protein synthesis
Removable auxiliary

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Medicine
Molecular Biology
Pharmacology
Drug Discovery
Organic Chemistry

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10.1002/psc.1336

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abstract = "Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.",

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AU - Spasser, Liat

AU - Ajish Kumar, K. S.

AU - Brik, Ashraf

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N2 - Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

AB - Side-chain assisted ligation is an auxiliary-mediated ligation strategy in which a thiol bearing cyclohexane or cyclopentane is attached to the side-chain of Asp, Glu, Ser or Thr to function in a similar manner to Cys in a native chemical ligation. Following the ligation step, the auxiliary is removed, without product isolation, under alkaline conditions.

KW - Aminoacyl transfer

KW - Native chemical ligation

KW - Peptide ligation

KW - Protein synthesis

KW - Removable auxiliary

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Scope and limitation of side-chain assisted ligation

Abstract

Keywords

ASJC Scopus subject areas

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