TY - JOUR
T1 - Screening for Dmrt genes from embryo to mature Macrobrachium rosenbergii prawns
AU - Amterat Abu Abayed, Faiza
AU - Manor, Rivka
AU - Aflalo, Eliahu D.
AU - Sagi, Amir
N1 - Funding Information:
We thank Dr. Vered Chalifa-Caspi and Dr. Menachem Sklarz for their help with the bioinformatics analyses, and the Dor Research Center for housing and maintaining the experimental prawns. This study was supported in part by the Israel Science Foundation (ISF) within the ISF-UGC joint research program (grant No. 2728/16 ).
Publisher Copyright:
© 2019 Elsevier Inc.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - The doublesex and mab-3 related transcription factor (Dmrt) gene family is known to be related to the sexual regulators doublesex of arthropods and mab-3 of annelids and to hold highly conserved functions in sexual determination and differentiation across phyla. Here, we report a study of the Dmrt gene family in the freshwater prawn Macrobrachium rosenbergii, a crustacean whose sexual differentiation has been widely researched. A wide transcriptomic screen, from the embryo to the adult M. rosenbergii, identified five novel Dmrt genes (MroDmrts) and confirmed two known MroDmrts. The seven MroDmrts encode proteins of 275–855 amino acids; each protein contained at least one conserved DNA-binding DM domain, which is typical of Dmrt proteins, and five proteins contained 1–4 transactivation domains (TADs). Importantly, in the embryonic, larval and post-larval stages, MroDmrt genes exhibited time-dependent expression patterns rather than sex-specific expression. In-silico screening of the expression of the MroDmrt genes in adult males revealed the enrichment of MroiDmrt1b and MroiDmrt1c in the androgenic gland (AG) as compared to the eyestalks. In vivo silencing of the androgenic gland insulin-like (IAG) encoding gene significantly decreased the expression of the above two Dmrt genes, while not affecting the expression of control genes, thereby suggesting the possible role of these two genes in the IAG-switch and in sex-differentiation processes.
AB - The doublesex and mab-3 related transcription factor (Dmrt) gene family is known to be related to the sexual regulators doublesex of arthropods and mab-3 of annelids and to hold highly conserved functions in sexual determination and differentiation across phyla. Here, we report a study of the Dmrt gene family in the freshwater prawn Macrobrachium rosenbergii, a crustacean whose sexual differentiation has been widely researched. A wide transcriptomic screen, from the embryo to the adult M. rosenbergii, identified five novel Dmrt genes (MroDmrts) and confirmed two known MroDmrts. The seven MroDmrts encode proteins of 275–855 amino acids; each protein contained at least one conserved DNA-binding DM domain, which is typical of Dmrt proteins, and five proteins contained 1–4 transactivation domains (TADs). Importantly, in the embryonic, larval and post-larval stages, MroDmrt genes exhibited time-dependent expression patterns rather than sex-specific expression. In-silico screening of the expression of the MroDmrt genes in adult males revealed the enrichment of MroiDmrt1b and MroiDmrt1c in the androgenic gland (AG) as compared to the eyestalks. In vivo silencing of the androgenic gland insulin-like (IAG) encoding gene significantly decreased the expression of the above two Dmrt genes, while not affecting the expression of control genes, thereby suggesting the possible role of these two genes in the IAG-switch and in sex-differentiation processes.
KW - Crustacea
KW - Dmrt
KW - Embryo
KW - IAG
KW - Larva and post larvae transcriptomic libraries
KW - Macrobrachium rosenbergii
KW - Transactivation domain (TAD)
KW - Zinc finger
UR - http://www.scopus.com/inward/record.url?scp=85068161123&partnerID=8YFLogxK
U2 - 10.1016/j.ygcen.2019.06.009
DO - 10.1016/j.ygcen.2019.06.009
M3 - Article
C2 - 31201800
AN - SCOPUS:85068161123
SN - 0016-6480
VL - 282
JO - General and Comparative Endocrinology
JF - General and Comparative Endocrinology
M1 - 113205
ER -