Screening of Rubiaceae and Apocynaceae extracts for mosquito larvicidal potential

Rahul Suryawanshi, Chandrashekhar Patil, Hemant Borase, Chandrakant Narkhede, Satish Patil

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Rubiaceae and Apocynaceae families are well known for the expression of cyclotides having insecticidal properties. Leaves and flowers extracts of plants from the families Rubiaceae (Ixora coccinea) and Apocynaceae (Allamanda violacea) were evaluated for mosquito larvicidal effect against early IVth instars of Aedes aegypti and Anopheles stephensi. Two forms of plant extracts, one untreated and the other treated with heat and proteolytic enzyme were used for assay. After primary assay, the extract showing more than 50% inhibition was further used for quantification purpose. LC50 and LC90 values of all the extracts were found to be reduced with the treated form. Phytochemical analysis of plant extracts was performed. Primary confirmation for the presence of cyclotides was done by Lowry test, thin layer chromatography and haemolytic assay. This novel approach merits use of plant extracts in mosquito control programmes.

Original languageEnglish
Pages (from-to)353-358
Number of pages6
JournalNatural Product Research
Issue number4
StatePublished - 16 Feb 2015
Externally publishedYes


  • Aedes aegypti
  • Anopheles stephensi
  • Apocynaceae
  • Rubiaceae
  • cyclotides
  • larvicidal

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry


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