Selective and universal primers for trematode barcoding in freshwater snails

J. Routtu, D. Grunberg, R. Izhar, Y. Dagan, Y. Guttel, M. Ucko, F. Ben-Ami

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Trematodes are significant pathogens of high medical, veterinary, and environmental importance. They are hard to isolate from their intermediate hosts, and their early life stages are difficult to identify morphologically. Therefore, primers were developed for trematodes to create a species barcoding system and allow selective PCR amplification in mixed samples. The specific oligonucleotide primer was universal for trematodes that infected several freshwater snail species in Israel. The diagnostic tool is based on the 18S rDNA gene. In contrast to morphological identification, trematode barcoding is rapid as it is based on a sequence of only 800 bp, and it classifies species accurately due to high polymorphism between conserved areas.

Original languageEnglish
Pages (from-to)2535-2540
Number of pages6
JournalParasitology Research
Volume113
Issue number7
DOIs
StatePublished - 1 Jan 2014
Externally publishedYes

Keywords

  • 18S rDNA
  • Centrocestus formosanus
  • Collyriclum faba
  • Flukes
  • Haplorchis pumilio
  • Haplorchis taichui
  • Lecithodendriidae sp
  • Lissorchus kritskyi
  • Opecoelidae sp
  • Paratanaisia bragai
  • Philophthalmus sp
  • Pleurogenes claviger
  • Sanguinicola cf. inermis
  • Transversotrema haasi

ASJC Scopus subject areas

  • Parasitology
  • Veterinary (all)
  • Insect Science
  • Infectious Diseases

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