Self-recognition mechanism of mamA, a magnetosome-associated TPR-containing protein, promotes complex assembly

Natalie Zeytuni, Ertan Ozyamak, Kfir Ben-Harush, Geula Davidov, Maxim Levin, Yair Gat, Tal Moyal, Ashraf Brik, Arash Komeili, Raz Zarivach

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

The magnetosome, a biomineralizing organelle within magnetotactic bacteria, allows their navigation along geomagnetic fields. Magnetosomes are membrane-bound compartments containing magnetic nanoparticles and organized into a chain within the cell, the assembly and biomineralization of magnetosomes are controlled by magnetosome-associated proteins. Here, we describe the crystal structures of the magnetosome-associated protein, MamA, from Magnetospirillum magneticumAMB-1 and Magnetospirillum gryphiswaldense MSR-1. MamA folds as a sequential tetratrico-peptide repeat (TPR) protein with a unique hook-like shape. Analysis of the MamA structures indicates two distinct domains that can undergo conformational changes. Furthermore, structural analysis of seven crystal forms verified that the core of MamA is not affected by crystallization conditions and identified three protein-protein interaction sites, namely a concave site, a convex site, and a putative TPR repeat. Additionally, relying on transmission electron microscopy and size exclusion chromatography, we show that highly stable complexes form upon MamA homooligomerization. Disruption of the MamA putative TPR motif or N-terminal domain led to protein mislocalization in vivo and prevented MamA oligomerization in vitro. We, therefore, propose that MamA self-assembles through its putative TPR motif and its concave site to create a large homooligomeric scaffold which can interact with other magnetosome-associated proteins via the MamA convex site. We discuss the structural basis for TPR homooligomerization that allows the proper function of a prokaryotic organelle.

Original languageEnglish
Pages (from-to)E480-E487
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number33
DOIs
StatePublished - 16 Aug 2011

ASJC Scopus subject areas

  • General

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