Sensitive enzymatic determination of neurotransmitters in artificial sweat

Itay Algov, Aviv Feiertag, Rafi Shikler, Lital Alfonta

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Dopamine (DA) and epinephrine (EN) are two phenolic molecules that are used in the human body and secreted in the brain actuating as neurotransmitters. As both molecules are highly important in the brain and the central nervous system, monitoring of their concentrations would enable better understanding of their importance and role in different physiological conditions. Copper efflux oxidase from Escherichia coli was shown in the past to have the ability of oxidizing polyphenolic compounds. As such, we have engineered this enzyme for its site-specific attachment to an electrode for the detection of DA and EN in high resolution and sensitivity. Here we present an enzymatic biosensor that enables the detection of such molecules with a 10 nM resolution and a linear range of up to 100 nM in artificial sweat samples. The presented biosensor could be used for the determination of catecholamines in different bodily fluids.

Original languageEnglish
Article number114264
JournalBiosensors and Bioelectronics
Volume210
DOIs
StatePublished - 15 Aug 2022

Keywords

  • Catecholamine sensor
  • Copper efflux oxidase (CueO)
  • Dopamine sensing
  • Epinephrine sensing
  • Site-specific wiring
  • Unnatural amino acids (UAAs)

ASJC Scopus subject areas

  • Biophysics
  • Biotechnology
  • Biomedical Engineering
  • Electrochemistry

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