Separation of human lymphocytes on Ficoll-Paque gradients: Stimulation of cells and depletion of a concanavalin-A responsive radioresistant subpopulation

R. Kol, M. Friedlander, E. Riklis, D. Raveh

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A subpopulation of human lymphocytes separated on Ficoll-Paque gradients showed an ultrastructural phenotype characteristic of stimulated cells. Thymidine incorporation was increased fivefold compared with unseparated (Buffy coat) controls. The Ficoll-Paque separated lymphocytes were more sensitive to γ radiation than unseparated lymphocytes and showed a decreased capability to undergo transformation in response to concanavalin A. Transformation in response to phytohemagglutinin and pokeweed mitogen was the same as for unseparated control lymphocytes. These results are interpreted as the selective depletion of a Con A-responsive T-cell fraction by Ficoll-Paque separation.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalRadiation Research
Volume95
Issue number1
DOIs
StatePublished - 1 Jan 1983

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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