Abstract
Using the fluorescence-activated cell sorter (FACS II), we have analyzed the expression of H-2K- and H-2D-gene products on the membrane of various cellular components of the murine immune system. Using this serological technique we show a basic difference between T and B lymphocytes. Whereas all cellular components analyzed - hydrocortisone-resistant thymocytes, splenic T and B lymphocytes, macrophages and bone-marrow cells - expressed H-2K-subregion-encoded alloantigens at a high density, it seems that the high density expression of H-2D-encoded alloantigens is restricted mainly to B cells and to macrophages. Hydrocortisone-resistant thymocytes, splenic T lymphocytes and bone-marrow cells, on the other hand, showed significant expression of the H-2D alloantigens only at low membrane density. These results, then, provide evidence for the existence of an imbalance in serologically detectable expression of H-2K- and H-2D-region-gene products on the cell membrane of various cells comprising the murine immune system.
Original language | English |
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Pages (from-to) | 585-595 |
Number of pages | 11 |
Journal | Immunogenetics |
Volume | 11 |
Issue number | 1 |
DOIs | |
State | Published - 1 Dec 1980 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology
- Genetics