Serum metabonomics coupled with Ingenuity Pathway Analysis characterizes metabolic perturbations in response to hypothyroidism induced by propylthiouracil in rats

Si Wu, Yue Gao, Xin Dong, Guangguo Tan, Wuhong Li, Ziyang Lou, Yifeng Chai

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A serum metabonomic profiling method based on ultra-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC/TOF-MS) was applied to investigate the metabolic changes in hypothyroid rats induced by propylthiouracil (PTU). With Significance Analysis of Microarray (SAM) for classification and selection of biomarkers, 13 potential biomarkers in rat serum were screened out. Furthermore, Ingenuity Pathway Analysis (IPA) was introduced to deeply analyze unique pathways of hypothyroidism that were primarily involved in sphingolipid metabolism, fatty acid transportation, phospholipid metabolism and phenylalanine metabolism. Our results demonstrated that the metabonomic approach integrating with IPA was a promising tool for providing a novel methodological clue to systemically dissect the underlying molecular mechanism of hypothyroidism.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume72
DOIs
StatePublished - 18 Jan 2013
Externally publishedYes

Keywords

  • Hypothyroidism
  • Ingenuity Pathway Analysis
  • Metabonomics
  • Propylthiouracil
  • Ultra performance liquid chromatography-mass spectrometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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