Serum microRNAs are promising novel biomarkers

Shlomit Gilad, Eti Meiri, Yariv Yogev, Sima Benjamin, Danit Lebanony, Noga Yerushalmi, Hila Benjamin, Michal Kushnir, Hila Cholakh, Nir Melamed, Zvi Bentwich, Moshe Hod, Yaron Goren, Ayelet Chajut

Research output: Contribution to journalArticlepeer-review

1116 Scopus citations

Abstract

Background: Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. Methods and Results: After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. Conclusions and Significance: Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers.

Original languageEnglish
Article numbere3148
JournalPLoS ONE
Volume3
Issue number9
DOIs
StatePublished - 5 Sep 2008
Externally publishedYes

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