Set-ups to induce by light a memory in in-vitro neuronal cultures

Clara Zaccaria; Mattia Mancinelli; Beatrice Vignoli; Ilya Auslender; Paolo Bettotti; Asiye Malkoç; Marco Canossa; Lorenzo Pavesi

doi:10.1117/12.2606876

Set-ups to induce by light a memory in in-vitro neuronal cultures

Clara Zaccaria, Mattia Mancinelli, Beatrice Vignoli, Ilya Auslender, Paolo Bettotti, Asiye Malkoç, Marco Canossa, Lorenzo Pavesi

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

To study the brain and the related neuronal network activity, many attempts were made to design and develop platforms able to induce and record neuronal signals. However, many brain processes - like memory formation and storage - and diseases - like amnesia or epilepsy - need more basic studies. For these, a bottom-up approach is needed, starting from 2D in-vitro neuronal cultures. In this work, we will present two experimental systems able to optogenetically interact with 2D neuronal networks with patternized light. One system consists in a Digital Light Projector (DLP) integrated in a microscope setup, which can illuminate neurons from the top; the other, is a compact and transportable photonic chip, properly designed to illuminate neurons plated on its surface.

Original language	English
Title of host publication	Optogenetics and Optical Manipulation 2022
Editors	Samarendra K. Mohanty, Anna W. Roe, Shy Shoham
Publisher	SPIE
ISBN (Electronic)	9781510647657
DOIs	https://doi.org/10.1117/12.2606876
State	Published - 1 Jan 2022
Externally published	Yes
Event	Optogenetics and Optical Manipulation 2022 - San Francisco, United States Duration: 22 Jan 2022 → 23 Jan 2022

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	11947
ISSN (Print)	1605-7422

Conference

Conference	Optogenetics and Optical Manipulation 2022
Country/Territory	United States
City	San Francisco
Period	22/01/22 → 23/01/22

Keywords

chip
digital
hybrid
interface
light
neural
photonic
projection

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2606876

Cite this

Zaccaria, C., Mancinelli, M., Vignoli, B., Auslender, I., Bettotti, P., Malkoç, A., Canossa, M., & Pavesi, L. (2022). Set-ups to induce by light a memory in in-vitro neuronal cultures. In S. K. Mohanty, A. W. Roe, & S. Shoham (Eds.), Optogenetics and Optical Manipulation 2022 [1194703] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11947). SPIE. https://doi.org/10.1117/12.2606876

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abstract = "To study the brain and the related neuronal network activity, many attempts were made to design and develop platforms able to induce and record neuronal signals. However, many brain processes - like memory formation and storage - and diseases - like amnesia or epilepsy - need more basic studies. For these, a bottom-up approach is needed, starting from 2D in-vitro neuronal cultures. In this work, we will present two experimental systems able to optogenetically interact with 2D neuronal networks with patternized light. One system consists in a Digital Light Projector (DLP) integrated in a microscope setup, which can illuminate neurons from the top; the other, is a compact and transportable photonic chip, properly designed to illuminate neurons plated on its surface.",

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note = "Funding Information: The BACKUP project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 788793). Funding Information: The BACKUP project received funding from the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation programme (grant agreement No 788793). Publisher Copyright: {\textcopyright} 2022 SPIE; Optogenetics and Optical Manipulation 2022 ; Conference date: 22-01-2022 Through 23-01-2022",

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Zaccaria, C, Mancinelli, M, Vignoli, B, Auslender, I, Bettotti, P, Malkoç, A, Canossa, M & Pavesi, L 2022, Set-ups to induce by light a memory in in-vitro neuronal cultures. in SK Mohanty, AW Roe & S Shoham (eds), Optogenetics and Optical Manipulation 2022., 1194703, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 11947, SPIE, Optogenetics and Optical Manipulation 2022, San Francisco, United States, 22/01/22. https://doi.org/10.1117/12.2606876

Set-ups to induce by light a memory in in-vitro neuronal cultures. / Zaccaria, Clara; Mancinelli, Mattia; Vignoli, Beatrice et al.

Optogenetics and Optical Manipulation 2022. ed. / Samarendra K. Mohanty; Anna W. Roe; Shy Shoham. SPIE, 2022. 1194703 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 11947).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Malkoç, Asiye

AU - Canossa, Marco

AU - Pavesi, Lorenzo

N1 - Funding Information: The BACKUP project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement No 788793). Funding Information: The BACKUP project received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement No 788793). Publisher Copyright: © 2022 SPIE

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N2 - To study the brain and the related neuronal network activity, many attempts were made to design and develop platforms able to induce and record neuronal signals. However, many brain processes - like memory formation and storage - and diseases - like amnesia or epilepsy - need more basic studies. For these, a bottom-up approach is needed, starting from 2D in-vitro neuronal cultures. In this work, we will present two experimental systems able to optogenetically interact with 2D neuronal networks with patternized light. One system consists in a Digital Light Projector (DLP) integrated in a microscope setup, which can illuminate neurons from the top; the other, is a compact and transportable photonic chip, properly designed to illuminate neurons plated on its surface.

AB - To study the brain and the related neuronal network activity, many attempts were made to design and develop platforms able to induce and record neuronal signals. However, many brain processes - like memory formation and storage - and diseases - like amnesia or epilepsy - need more basic studies. For these, a bottom-up approach is needed, starting from 2D in-vitro neuronal cultures. In this work, we will present two experimental systems able to optogenetically interact with 2D neuronal networks with patternized light. One system consists in a Digital Light Projector (DLP) integrated in a microscope setup, which can illuminate neurons from the top; the other, is a compact and transportable photonic chip, properly designed to illuminate neurons plated on its surface.

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Set-ups to induce by light a memory in in-vitro neuronal cultures

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this