Significant catalytic roles for Glu47 and Gln 110 in all four of the C-C bond-making and -breaking steps of the reactions of acetohydroxyacid synthase II

Keyphrases

• C-C Bond 100%
• Acetohydroxyacid Synthase 100%
• Carboligation 100%
• Catalytic Role 100%
• Thiamine Diphosphate 75%
• Donor Substrate 75%
• Hydrogen Bonds (H-bonds) 50%
• Glutamine 50%
• Thiamine Diphosphate-dependent Enzymes 50%
• Substrate Binding 50%
• α-keto Acids 50%
• Acceptor Substrate 50%
• Steady State 25%
• Escherichia Coli 25%
• Active Sites 25%
• Proton Shuttle 25%
• Side Chain 25%
• Minor Effect 25%
• High Specificity 25%
• Glutamate 25%
• Carbonyl 25%
• Isozymes 25%
• Acetoacetate 25%
• Protonation 25%
• Steady-state Kinetics 25%
• Histidine 25%
• Tautomerization 25%
• General Acid 25%
• Polar Residues 25%
• Hydroxyethyl 25%
• Carboxylate 25%
• Bond Distance 25%
• Bond Breaking 25%
• Elementary Reaction 25%
• Branched-chain Amino Acids 25%
• Acid Condensation 25%
• α-ketobutyrate 25%
• Transketolase 25%
• Oxyanions 25%
• Transient Kinetics 25%
• Acid-base Catalyst 25%
• Enamine Intermediate 25%
• Benzaldehyde Lyase 25%
• Substrate Competition 25%
• Product Elimination 25%

Biochemistry, Genetics and Molecular Biology

• Thiamine Pyrophosphate 100%
• Acetolactate Synthase 100%
• Enzyme 60%
• Steady State 40%
• Glutamine 40%
• Anabolism 20%
• Amino Acids 20%
• Escherichia coli 20%
• Isozyme 20%
• Kinetics 20%
• Protonation 20%
• Active Site 20%
• Histidine 20%
• Tautomerization 20%
• Lyase 20%
• Transketolase 20%