Simkania negevensis strain Z(T): Growth, antigenic and genome characteristics

Simona Kahane, Karin D.E. Everett, Nina Kimmel, Maureen G. Friedman

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52 Scopus citations

Abstract

Simkania negevensis is the type species of Simkaniaceae, a recently proposed family in the order Chlamydiales. In the current study, growth, antigenic and genomic characteristics of this intracellular bacterium were investigated and compared to those of members of the family Chlamydiaceae. Growth of the organism, as assessed by infectivity assays, reached a plateau in 2-3 d although by light microscopy the cytopathic effect on the host cells increased for 12 or more days after infection. S. negevensis growth was unaffected by sulfadiazine. Cells infected by S. negevensis strain Z(T) were not recognized by either of two monoclonal antibodies specific for Chlamydiaceae LPS and several specific Chlamydiaceae ompA primers were unable to PCR amplify a S. negevensis gene. The S. negevensis genome contained one copy of the ribosomal operon. The genome size of S. negevensis strain Z(T) was determined by PFGE to be 1.7 Mbp, and the G+C content was 42.5 mol%. These data, taken together with other published data, are consistent with the proposal that S. negevensis belongs to a distinct family in the order Chlamydiales.

Original languageEnglish
Pages (from-to)815-820
Number of pages6
JournalInternational Journal of Systematic Bacteriology
Volume49
Issue number2
DOIs
StatePublished - 1 Jan 1999

Keywords

  • Chlamydia
  • Chlamydiales
  • Genome size
  • Simkaniaceae
  • rDNA copy number

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